Reagent Preparation Sheet.

1 X Phospate buffered saline, 0.15 M, pH 7.6, 0.05 % Tween 20

Washing and resuspension buffer, working solution

Date prepared:

By:

  1. Hazard Assessment

    To protect yourself from any possible hazards associated with this task wear eye protection. You should also wear latex, nitrile, or vinyl gloves and a lab coat with long sleeves. To protect your legs and feet wear closed shoes and long trowsers. Do not wear sandles, shorts or a short skirt. Wash your hands before eating and when leaving the laboratory. You should review the MSDS for any chemical used in this procedure. In case of a spill with a toxic chemical remove all contaminated clothing and wash affected areas with copious quantities of water. Check location of the nearest safety shower. Eyes should be washed copiously for 15 minutes.

  2. Reagent
    1 X Phospate buffered saline, pH 7.6, 0.05 % Tween, (NaCl xmM, KCl xmM, Na2HPO4.7H20 xmM, KH2P04 xmM)

  3. Purpose of reagent
    Used for all washes in ELISA protocol and as a diluent for Streptavidin horseradish peroxidase.

  4. Recipe
    ReagentAmountAdded
    1 X PBS1000 ml ____
    Tween 200.05 ml ____

  5. Comments

    Measure out 1000 ml of 1 X PBS in a 1 liter graduate cylinder. Add the Tween by pipette drop by drop. Cover the cylinder with parafilm and invert it to mix the Tween. Pour into a reagent bottle and label "SeM PBST" with the date.
  6. Reference
    Short Protocols in Molecular Biology, page A1-44