Reagent Preparation Sheet.

0.15M PBS, pH 7.6, coating buffer

ELISA Coating Buffer

Date prepared:

By:

  1. Hazard Assessment

    To protect yourself from any possible hazards associated with this task wear eye protection. You should also wear latex, nitrile, or vinyl gloves and a lab coat with long sleeves. To protect your legs and feet wear closed shoes and long trowsers. Do not wear sandles, shorts or a short skirt. Wash your hands before eating and when leaving the laboratory. You should review the MSDS for any chemical used in this procedure. In case of a spill with a toxic chemical remove all contaminated clothing and wash affected areas with copious quantities of water. Check location of the nearest safety shower. Eyes should be washed copiously for 15 minutes.

  2. Buffer
    0.15M PBS, pH 7.6, coating buffer

  3. Purpose of buffer
    Basis buffer in SeM ELISA protocol for coating plates with MAB212p, also the 1X PBS used in blocking, washing and antibody dilution.

  4. Buffer recipe

    ReagentAmountAdded
    sodium chloride8.0 g ____
    Potassium chloride0.2 g ____
    KH2PO40.2 g ____
    Na2HPO4.7H202.17 g ____
    water 900 ml ____
    Adjust pH to 7.6 with 6N HCL or 5N NaOH as necessary____
    Add water to 1 liter____

  5. Comments
  6. Reference
    Provided by Nathalie Forster (Maine Biotechnology Services) from their ELISA protocol for SeM