1 X Phospate buffered saline, pH 7.6, 1% Non fat Dried Milk
Blocker
By:
To protect yourself from any possible hazards associated with this task wear eye protection. You should also wear latex, nitrile, or vinyl gloves and a lab coat with long sleeves. To protect your legs and feet wear closed shoes and long trowsers. Do not wear sandles, shorts or a short skirt. Wash your hands before eating and when leaving the laboratory. You should review the MSDS for any chemical used in this procedure. In case of a spill with a toxic chemical remove all contaminated clothing and wash affected areas with copious quantities of water. Check location of the nearest safety shower. Eyes should be washed copiously for 15 minutes.
Phospate buffered saline, 0.15 M, pH 7.6, 1 % skim milk.
Blocking agent in SeM ELISA.
| Reagent | Amount | Added |
| Coating buffer (PBS 0.15 M, pH 7.6) | 100 ml | ____ |
| Non-fat dried milk (NFDM) | 1 g | ____ |
Measure out 100 ml of coating buffer (0.15 M PBS, pH 7.6) in a 100 ml graduate cylinder, pour into a 125 ml DIFCO bottler. Drop in a stir bar, agitate, and slowly add 1 g of NFDM. Cap the bottle and label "Blocker PBS, 1% NFDM" with the date. Store at 4 C. Do not use past one week.