Working dilution of MAB212p in coating buffer for coating ELISA plates at a concentration of 2 ug/mL
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MAB212p dilution in coating buffer for SeM ELISA
Serves as the capture antibody MAB212p for SeM ELISA. This antibody serves as the coating agent on the ELISA plate to concentrate target SeM and Sem containing streptococci.
5 Vials (1 mL) received at 1.4mg/mL. Aliquotted into 50 ul aliquots and frozen at -20 C.
Number of moles in 10 mL @ 2 ug/mL = Number of moles in x mL @ 1.4 mg/ml (added to 10 mL buffer)
10 mL X 2 ug/mL = x mL X 1.4 mg/mL
10 mL X 2 ug/mL = x mL X 1400 ug/mL
x mL = (10 mL X 2 ug/mL)/ 1400ug/mL solve for x
x ul = 1000 *(10 mL X 2 ug/mL)/1400ug/mL give volume in uL
x ul = (10 mL X 2 ug/mL)/1.4 mg/mL 1000's cancel out
x = 10*2/1.4 yields volume (in uL) of MAB212p @ 1.4 mg/mL to add to 10 mL to make a 2 ug/mL solution in uL
The R code to generate table below
> y=c(1.2,1.3,1.4,1.5) # a variety of concentrations, including the 1.4 mg/mL used in the example
> x=10*2/y; x #prints volumes (uL) to add of each concentration
[1] 16.66667 15.38462 14.28571 13.33333
| Original concentration MAB212p | 1.2 mg/mL | 1.3 mg/mL | 1.4 mg/mL | 1.5 mg/mL |
| Volume MAB212p to use | 16 ul | 15 ul | 14 uL | 13 ul |
| Volume of coating buffer (0.15M PBS, pH 7.6) | 10 mL | 10 mL | 10 mL | 10 mL |
| Check which volume used | ____ | ____ | ____ | ____ |