Reagent Preparation Sheet.

Buffer PE (10 mM Tris-HCl pH 7.5) in SeM PCR

Date prepared:

By:

  1. Hazard Assessment

    To protect yourself from any possible hazards associated with this task wear eye protection. You should also wear latex, nitrile, or vinyl gloves and a lab coat with long sleeves. To protect your legs and feet wear closed shoes and long trowsers. Do not wear sandles, shorts or a short skirt. Wash your hands before eating and when leaving the laboratory. You should review the MSDS for any chemical used in this procedure. In case of a spill with a toxic chemical remove all contaminated clothing and wash affected areas with copious quantities of water. Check location of the nearest safety shower. Eyes should be washed copiously for 15 minutes.

  2. Reagent
    Buffer PE (10 mM Tris-HCl, pH 7.5) in 95% ethanol for SeM PCR protocol

  3. Purpose of reagent
    Serves as a wash buffer for the clean-up of DNA products

  4. To Prepare a Working Dilution

  5. Comments
  6. Reference
    Genelute Overview

    Genelute technical bulletin